Fig. 2. The ER-Golgi transport container (EGTC) is an isolated, membrane-bound entity. (A) Transmission electron micrographs of six serial 70 nm sections of VSV-infected NRK cells subjected to the rapid ethane-freezing procedure 10 minutes after release from 40°C. An EGTC is shown with the surrounding membranes in view, demonstrating the lack of membranous connection between the EGTC and the surrounding membranes. Bar, 0.1 µm. (B) Three-dimensional reconstruction of the serial sections in (A) using a contour alignment reconstruction (CAR) program (Saetzler et al., 2002) viewed from two different angles. The spheres are projections of the vesicular profiles at the edges of the EGTC. Scale: 1 mm represents 10 nm.