Fig. 1. The effects of increasing doses of an RGD-containing fibronectin fragment and IGFBPs on the adhesion of Hs578T cells to ECM. The graphs show the percentage of cells attached to ECM gel. Cells were treated with (A) RGD (0-100 µg/ml), RGD (10 µg/ml) or RGE (10µg/ml) and RGD (10 µg/ml) with (B) IGFBP-2 (50-800 ng/ml), (C) IGFBP-3 (0-100 ng/ml), (D) IGFBP-5 (0-100 ng/ml) or (E) IGFBP-4 (100 ng/ml) and (F) IGFBP-1 (100 ng/ml) or IGFBP-6 (100 ng/ml). Where ^* is considered statistically significant relative to the control in all experiments as defined by Bonferroni/Dunn post-hoc test. Data represent the mean of three experiments each in triplicate±standard error of the mean (s.e.m.).