Fig. 2. The effects of the IGFBPs on the adhesion of Hs578T cells to laminin, collagen type IV and fibronectin. The graphs show the percentage of cells attached. Cells were treated with RGD (10 µg/ml), IGFBP-1 (100 ng/ml), IGFBP-2 (100 ng/ml) IGFBP-3 (100 ng/ml), IGFBP-4 (100 ng/ml), IGFBP-5 (100 ng/ml) and IGFBP-6 (100 ng/ml) before plating on either laminin (5 µg/ml) (A-C), collagen type IV (0.25 µg/ml) (D-F) or fibronectin (0.25 µg/ml) (G-I). ^* indicates a statistically significant difference relative to the control in each experiments as defined by Bonferroni/Dunn post-hoc test. Data represent the mean of three experiments each in triplicate±s.e.m.