Fig. 5. The effects an RGD-containing fibronectin fragment on the actions of IGFBP-3 and -5 on C2-induced apoptosis. The graphs show the percentage of dead cells. Cells were treated with either (A) IGFBP-3 (100 ng/ml) or (B) IGFBP-5 (100 ng/ml) ± a non-apoptotic dose of RGD (10 µg/ml) for 24 hours followed by a co-incubation with an apoptotic dose of C2-ceramide. ^* indicates a statistically significant difference relative to the control in each experiments as defined by Bonferroni/Dunn post hoc test. Data represent the mean of three experiments each in triplicate±s.e.m.