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Fig. 5. Cell viability after hypo-osmotic shock (OS). Cells were assayed for metabolic activity (CellTiter 96, Promega) at different time intervals of recovery after 5 minutes exposure to 150 mM urea. Metabolic activity is presented as a percentage of the activity in the untreated control cell population. Wild-type cell cultures returned to starting levels of metabolic activity after 2 days; KEB-4 reached this level in 6 days, and KEB-7 cultures showed no recovery in 8 days.