Fig. 3. Identification of Ned1 protein. (A) Extracts prepared from a wild-type (a) or a ned1 strain (b) were examined by western blot analysis. Numbers on the left indicate the molecular weight (kDa) of protein standards. (B) Wild-type fission yeast cell extracts prepared from a logarithmic (a) or from a stationary culture (b) in EMM2 at 30°C. Each of the protein samples was digested with alkaline phosphatase in the absence (-) or presence (+) of phosphatase inhibitors. Numbers on the left indicate the molecular weight (kDa) of each band. (C) Protein samples prepared from a synchronized culture at indicated times are analyzed by western blotting (top). The arrow indicates the position of a 90 kDa band. The synchrony was shown by the profile of the septation index (bottom).