Fig. 6. Ned1-Dis3 interactions. (A) Strains indicated are incubated at 36°C or at 26°C for 3 days and at 22°C for 4 days. (B) Two-hybrid interaction between Ned1 (1-121) and Dis3 (927-970). The activity of Ned1 protein segment containing a substitution mutation G80A or G80R as well as blank plasmid is shown on the —Ade, His plate. (C) The rate of minichromosome loss per cell division was determined by the half-sectored colony assay (Allshire et al., 1995). In each experiment, approximately 3x10⁴ divisions were scored. The values are the average of two experiments.