Fig. 1. C₁-domain proteins that bind DAG and phorbol esters with high affinity. C1, protein kinase C conserved region 1; C2, protein kinase C conserved region 2 (CalB); DAG, diacylglycerol; DAGKa, diacylglycerol kinase accessory domain; DAGKc, diacylglycerol kinase catalytic domain; EF, EF hand; GAP, GTPase-activator protein; GEF, guanine nucleotide exchange factor; GRP, guanyl-releasing protein; PH, pleckstrin-homology domain; pKc, protein kinase C terminal domain; PKC, protein kinase C; PKD, protein kinase D; RasGEF, guanine nucleotide exchange factor for Ras-like small GTPases; RasGEFN, guanine nucleotide exchange factor for Ras-like GTPases (N-terminal motif); RhoGAP, GTPase-activator protein for Rho-like GTPases; S/T-Kc, serine/threonine protein kinases, catalytic domain; SH2, Src homology 2 domain.