Fig. 2. Nonspecific effects of a bisindolylmaleimide-derived PKC inhibitor in hippocampal neurons. (A) Time course of phorbol-ester effects on evoked EPSCs in wildtype neurons (n=6). Application of PDBU (1 µM) is indicated by the white box. (B) Preincubation with 3 µM bisindoylmaleimide I (Gö6857, grey box) led to a partially irreversible rundown of evoked EPSC amplitudes but did not block the potentiation induced by application of 1 µM PDBU (white box). (C) Average evoked EPSC amplitudes in untreated neurons before and after bisindoylmaleimide I (3 µM) pretreatment (n=6). (D) Average phorbol-ester-dependent potentiation of evoked EPSC amplitudes (30 seconds following onset of application of 1 µM PDBU, n=6) in untreated and bisindoylmaleimide I (3 µM) pretreated neurons from experiments shown in (B). Error bars indicate s.e.m.