Fig. 3. Neurons expressing a phorbol-ester-binding-deficient Munc13-1 are completely insensitive to phorbol esters. Time course of phorbol-ester effects on evoked EPSCs in hippocampal neurons from wild-type-like Munc13-2-deficient mice (black squares, n=6) and Munc13-2-deficient/Munc13-1^H567K double mutant mice (grey circles, n=6). Application of PDBU (1 µM) is indicated by the black bar. EPSCs were evoked at 0.2 Hz and normalised to the initial amplitude. Error bars indicate s.e.m.