Fig. 2. Association of Pol with the replication machinery. Cells were transfected with peGFP-C2/pol and treated for 12 hours with 1.5 mM HU (A,B). HU was maintained (A) or chased by rinsing cells with fresh medium and then incubated for 8 hours (B). Cells were fixed and stained with anti-PCNA mAb followed by TRITC-conjugated secondary antibody. The localisation of eGFP-pol (green) and PCNA (red) was observed in the same cell and the colocalisation is indicated by the yellow pattern (Merge). About 1000 fluorescent cells were analysed.