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Fig. 8. Transient expression of hARL5 or its mutants and HP1{alpha} in COS-7 cells. COS-7 cells co-transfected with Flag-tagged HP1-{alpha} and GFP-tagged constructs of hARL5, hARL5(Q80L), hARL5(T35N), hARL5(dC), hARL5(dMIR), or hARL5(Q80LdMIR) were grown on glass coverslips, fixed with formaldehyde, and incubated with mouse anti-Flag (d,h,l,p,t,x) or mouse anti-nucleolin (nucleolar marker) antibodies. Coverslips were mounted on Mowiol (supplemented with Hoechst 33258; b,f,j,n,r,v) and inspected with a Zeiss Axiophot equipped for epifluorescence. Phase-contrast microscopy (a,e,i,m, q,u) and GFP-hARL5 fluorescence (c,g,k,o,s,w; green) are shown. Nucleolins are indicated by arrows. Co-localization of HP1-{alpha} and hARL5(Q80L) is indicated by arrowheads. Bar, 10 µm.