Fig. 2. dSyx16 binds dSNAP in a concentration-dependent fashion. From lane 1 to 5, immobilized GST-dSyx16 or GST were incubated with 10, 4, 2, 0 and 10 µg of recombinant dSNAP, respectively. Proteins on the glutathione beads were then eluted and subjected to SDS-PAGE and western blot analysis.