Fig. 2. Subcellular localization of Cav-1 and Cav-2 by confocal analysis of Caco-2 cell clones. Cells expressing Cav-1 (a,c,e) or Cav-2 (b d,f) were double-labeled with rabbit polyclonal anti-Cav-1 (N20) or Cav-2 antibodies (red) and mouse monoclonal antibodies (green) to SI (a,b), Ag 525 (c,d) or Giantin (e,f), used as apical, basolateral or Golgi markers, respectively. Z optical sections (a,b) show that neither Cav-1 (a) nor Cav-2 (b) can be observed at the apical membrane stained for SI (arrowheads). Cav-1 (c,e) is detected both in the Golgi complex and at the lateral membrane (arrows) while Cav-2 (d,f) only showed a perinuclear labeling (arrows). Bar, 5 µm.