Fig. 4. Localization of Cav-2 in Caco-2 cells expressing Cav-1. (a) Confocal sections showed that Cav-1 and Cav-2 overlapped in a perinuclear region (arrowhead) with Cav-1 showing an additional cytoplasmic and lateral labeling (arrowhead). (b) Cav-2 was still restricted to the Golgi complex co-localizing with Giantin (arrows) in Cav-1 expressing Caco-2 cells. (c,d) Localization of GFP-Cav-2 or Cav-2-GFP (green), respectively, in Cav-1 Caco-2 cells labeled with an antibody against Cav-1 (red). No GFP staining of the plasma membrane could be detected (arrows) while accumulation into an intracellular compartment was observed (arrowheads). Bar, 5 µm.