Fig. 6. MURF2 associates with A-band titin in nascent myofibrils. C57 cells were stained with anti-titin A-band (T31, red) and anti-MURF2 (green) antibodies. After 24 hours of differentiation (A), titin aligned with MURF2 positive microtubules in a dotty pattern. After 48 hours of differentiation (B), titin A-band epitopes were organised in patches along MURF2 positive structures. At 120 hours of differentiation (C), MURF2 colocalises with titin A-band epitopes as longitudinally oriented dots (arrowhead). Some dots were observed at both sides of nascent striated myofibrils (arrow).