Fig. 7. MURF2 and sarcomeric myosin show colocalisation in premyofibrils. C57 cells were stained with anti-MURF2 (green) and with anti sarcomeric myosin heavy chain antibodies (red). Double immunofluorescence experiments revealed that MURF2 stained filamentous structures containing sarcomeric myosin at early stages of differentiation (A, arrowheads). Later, MURF2 and sarcomeric myosin colocalise in non-striated regions parallel to striated myofibrils (B, arrowheads). Upon sarcomere formation, MURF2 and sarcomeric myosin antibodies labelled identical rod-like structures (C, arrowheads). Mature skeletal sarcomeres (arrow) did not show colocalisation of MURF2 and sarcomeric myosin.