Fig. 6. Comparison of the distribution of VSVG-GFP with Golgi-resident proteins. BHK cells (a-c) or HeLa cells (d-i) were co-transfected with expression plasmids for VSVG-GFP and FLAG-RGSZ1. The cells were incubated for 20 hours at 40°C, and then the temperature was changed to 32°C. After 1 hour at 32°C, the cells were fixed and then stained with antibodies to Man II (b), ß-COP (e), or ERGIC-53 (h). Merged images are presented on the right (c,f,i). Bar, 10 µm.