Fig. 8. Expression of a dominant-negative G_z results in Golgi disruption. HeLa cells were transfected with wild-type G_z (a,b) or a triple mutant (G204A/E246A/A327S) (c,d). The cells were allowed to express G_z for 20 hours, fixed, and then double-stained with the anti-G_z antibody (a,c) and anti-membrin antibody (b,d). Arrows indicate cells overexpressing the mutated G_z with a dispersed Golgi apparatus. Bar, 20 µm.