Fig. 9. Golgi localization of G_z. BHK cells (a-j) and Clone9 cells (c-l) were fixed and then subjected to fluorescence microscopic analysis. The rabbit polyclonal anti-Gz antibody and mouse monoclonal anti-Man II antibody were used to visualize G_z (a,c,e,g,i,k) and Man II (b,d,f,h,j,l), respectively. In panels e-h, the anti-G_z antibody was preincubated with the peptide used for immunization. In panels i to l, cells were preincubated with 10 µM BFA for 10 minutes (i,j) or 30 minutes (k,l) before fixation.