Fig. 3. Expression of cadherins and catenins on isolated thymic epithelial cells. Isolated primary thymic epithelial cells cultured on chamber slides were fixed and stained with antibodies against E-cadherin (A), P-cadherin (B), ß-catenin (C) and -catenin (D) followed by Cy3^TM-labelled second antibodies. Control cultures were incubated only with the Cy3^TM-labelled anti-mouse (E) or anti-rabbit (F) antibodies. The cultured thymic epithelial cells showed expression of all four analysed antigens. Expression was mainly found on the cell surfaces at sites of cell-cell contacts. Bar, 100 µm.