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Fig. 1. Distribution of cPLA-{alpha} in confluent and subconfluent EA.hy.926 cells. (A) cPLA-{alpha} was detected by western blotting EA.hy.926 lysates (20 µg protein) using an affinity-purified goat polyclonal antibody (lane 1). Also shown are control lanes corresponding to antigen-adsorbed antibody (lane 2) and HRP anti-goat IgG controls (lane 3). (B) Cells (1x10 for subconfluent and 5x10 for confluent) were seeded onto glass coverslips, and cPLA-{alpha} was detected by immunofluorescence microscopy. Bar, 10 µm. (C) Densitometrical plot analysing the distribution of staining across individual cells (marked by dashed line in B). (D) Peptide-adsorbed antibody control. (E) Detection of NuMA in confluent and subconfluent cells.