Fig. 2. cPLA₂- in nuclear fractions. (A) Nuclei from subconfluent and confluent cells were isolated and analysed by immunofluorescence microscopy. Also shown are nuclei obtained from A23187-stimulated cells (5 µM, 10 minutes). (B) 20 µg of total homogenate and nuclear samples were western blotted, and LDH and cPLA- were detected using goat polyclonal antibodies. (C) Quantification of the amounts of cPLA- in nuclear fractions expressed as a percentage of the amount in the total sample±s.e.m. (n=3).