Fig. 4. Effects of staurosporine treatment on the nuclear localisation of cPLA-. Cells (1x10 for sub-confluent, 5x10 for confluent samples) were seeded onto coverslips and grown overnight. Cells were then treated with 1 µM staurosporine in HEPES/Tyrode's buffer for 30 minutes at 37°C (control cells were incubated with buffer alone). cPLA- was detected by immunofluorescence microscopy. Bar, 10 µm. (B) The intensity of cPLA- staining in sections through the nuclei of confluent (i) and subconfluent (ii) cells was quantified. Plots represent average intensities (in arbitrary units)±s.e.m. (n=90, data from three independent experiments).