Fig. 8. Effects of leptomycin B (LMB) on the nuclear export of cPLA-. Cells were grown overnight on coverslips then serum starved for 24 hours. The effects of LMB were examined by treating the cells for 1 hour with 10 ng/ml LMB prior to serum starving. (A) cPLA- was detected in control cells (a), serum-starved cells (b) and LMB-treated serum-starved cells (c). Bar, 10 µm. (B) Graph showing changes in intensity of nuclear staining (in arbitrary units, n=90, data represents averages from three independent experiments±s.e.m.) in sections taken through the nuclei of cells.