Fig. 6. The differentiation of lobed structures in mycelium from CBEL+
and CBEL- P.p.n. strains in contact with cellulosic
substrates. The mycelium was grown on a cellophane membrane (A,B) or on flax
cellulose fibres dispersed onto a cellophane membrane (C,D) placed on top of
V-8 agar medium. After 7 days of culture, the cellophane sheet was removed
from the medium underneath and processed for scanning electron microscopy.
(A,C) Aggregates of intricate ramified hyphae (arrows) were observed in the
EX11-1 CBEL+ strain grown on cellophane or flax fibres. (B,D) The
EX11-4 CBEL- strain formed only smooth hyphae that rarely branched
on either substrate. Asterisks indicate the flax cellulose fibres. Bars, 40
µm.