Fig. 2. Accumulation of SLBP during oocyte maturation. GV-stage oocytes were collected and incubated for the period of time indicated at the top of each lane, then immunoblotted using the anti-SLBP antiserum. All oocytes in the 0 hour group contained a GV; all oocytes in the other groups had undergone germinal vesicle breakdown. Oocytes in the 9 hour group were separated into those that had or had not emitted the first polar body (pb). All oocytes in the 12 and 24 hour groups had produced a polar body. Lanes 1 and 2 contain 50 oocytes; all other lanes contain 25 oocytes.