Fig. 4. Intracellular distribution of SLBP during early embryogenesis. (A) 1-cell. (B) 2-cell. (C1) Early 4-cell; DAPI stain to reveal DNA. The two lower nuclei appear to be at late telophase/early interphase. (C2) Early 4-cell; SLBP staining. (D1) 5- to 8-cell; DAPI stain. Five nuclei, one set of metaphase chromosomes and the DNA of the second polar body (arrow) are visible. (D2) 5- to 8-cell; SLBP staining. Long exposure reveals weakly stained nuclei and cytoplasm of embryo and strongly stained polar body (arrow). (Inset) Same embryo, exposure for the same duration as A-C.