Fig. 1. Geno- and phenotyping of selected keratinocyte cultures before and after conditional ß-catenin gene ablation by Adenovirus-encoded Cre-recombinase. (A) PCR performed on genomic DNA of cultured mouse keratinocytes depicts complete recombination of ß-cat^+loxP into ß-cat^- alleles in cultures 1-3. (B) Western blot analysis of whole cell lysates was used to confirm lack of ß-catenin expression in conditional null mutant keratinocytes. Plakoglobin was assessed on the same blot and used as a loading control. ß^-, deleted ß-catenin allele; ß^+loxP, transgenic allele carrying two loxP sites; ß⁺, wild-type allele; PG, plakoglobin.