Fig. 3. Cells that express markers characteristic for hypertrophic and/or
calcifying chondrocytes differentiated in EB outgrowths. Collagen X
mRNA (A,B), osteopontin protein (C,D) and bone sialoprotein I + II (E,F) were
expressed in cells organized in nodules (A,C,E) or as single cell clusters
(B,D,F) in EB outgrowths. Representative areas of EB outgrowths at 5+23 d (A),
5+16 d (B) and 5+30 d (C-F) are shown. Bar, 100 µm.