Fig. 6. Additional mesenchymal cell types developed in cultures of chondrogenic cells isolated from EBs. Adipocytes (A), skeletal muscle cells (B) and epithelial cells (C) were detected in cultures of isolated chondrocytes after 12-14 days as demonstrated by histochemical staining with Sudan III (A) and immunostaining for sarcomeric actinin (B) or a mixture of cytokeratins (C), respectively. Bar, 100 µm.