Fig. 7. Application of TGF-ß3 resulted in downregulation of chondrogenic marker genes in cultures of chondrogenic cells isolated from EBs. Semi-quantitative RT-PCR demonstrated that the transcription levels of the juvenile splice variant A (A) as well as the adult splice variant B (B) of collagen II, collagen X (C) and osteocalcin (D) significantly decreased after application of TGF-ß3 at two different concentrations of 10 and 50 ng/ml to cultures of chondrogenic cells isolated from EBs. The mRNA levels in relation to those of HPRT are shown. Mean values±s.e.m. derived from independent experiments (n=2) are presented. Significant differences (in comparison to untreated control cells): ^*P0.05; ^**P0.01; ^***P0.001.