Fig. 1. Aberrant morphology of rho3 null cells. (A) Growth of wild-type cells (WT) and rho3 null cells (rho3) on a YEA plate and on YEA containing 1 M sorbitol at the indicated temperatures for 3 days. (B) Wild-type cells and rho3 null cells grown in YEA medium at the indicated temperatures for 16 hours were stained with Calcofluor. (C) Wild-type cells and rho3 null cells grown in YEA medium at 25 or 37°C for 5 or 16 hours were stained with Rhodamine-phalloidin (red in merged images), TAT-1 (green in merged images), and DAPI (blue in merged images). Deconvoluted 3D images are shown. Arrows indicate cells with abnormal organization of cytoplasmic MTs. Arrowheads indicate nuclei in a cytokinesis-defective binucleate cell. Bars, 10 µm.