Fig. 5. Overexpression of For3. (A) Effects of overexpression of For3 or truncated proteins on cell viability. Wild-type cells or rho3 null cells containing indicated vectors, respectively, were grown on an EMM plate with (+) or without (-) thiamine at 25°C for 4 days. (B) Effect of For3 overexpression on cell shape. Wild-type cells containing pREP1for3 grown in EMM without thiamine for the indicated periods were stained with Calcofluor. Arrows indicate bulges. Arrowheads indicate swollen cells. (C) MT bundles were formed in For3-overexpressing cells. The cells (0 and 20 hours in B) were fixed and stained with Rhodamine-phalloidin (top) and TAT-1 (bottom). Deconvoluted 3D images are shown. Arrows indicate thick bundles of cytoplasmic MTs. Bars, 5 µm.