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Fig. 3. (A) Proliferation of cells adherent to LAP-TGFß1. Equal numbers of {alpha}8-transfected or mock-transfected AtT20 cells were plated on 5 µg/ml LAP-TGFß1 or 5 µg/ml fibronectin (FN) in serum-free media. After 3 days, proliferation was assayed using the Roche Cell Proliferation kit (MTT). Results for four independent clones of AtT20{alpha}8 cells are shown. Data is reported as the mean absorbance of triplicate wells±s.d. (B) ERK phosphorylation on LAP-TGFß1. {alpha}8-transfected or mock-transfected AtT20 cells were plated on 0.01% poly-L-lysine (PLL) 5 µg/ml fibronectin (FN), 5 µg/ml LAP-TGFß1 (LAP1), or 5 µg/ml RGE-LAP-TGFß1 (RGE) for 30 minutes in serum-free media and then lysed in buffer containing phosphatase inhibitors. Equal amounts of protein were loaded and probed with an antibody to phospho-ERK (top) or ERK (bottom).