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Fig. 4. (A) Adhesion of LAP-TGFß1 to {alpha}8ß1 does not activate TGFß1. Equal number of Mv1Lu reporter cells and test cells (SW480{alpha}8 or SW480ß6) were co-cultured for 16 hours with indicated concentrations of the integrin-activating antibody 8A2 and lysed for measurement of luciferase activity. Results are the means of at least two experiments done in duplicate. (B) {alpha}8-transfected cells do not affect TGF-ß1 activation by SW480ß6 cells. Mv1Lu reporter cells were cultured with SW480{alpha}8 cells and SW480ß6 cells, or mock-transfected SW480 cells and SW480ß6 cells, for 16 hours and lysed for measurement of luciferase activity. Results are the means of duplicate experiments±s.d. (C) Adhesion of LAP-TGFß3 to {alpha}8ß1 does not activate TGFß3. CHO or CHO{alpha}8 cells were transiently transfected with full-length expression construct of TGFß3. After 16 hours, transfected cells were cultured for 24 hours with Mv1Lu reporter cells. Cells were lysed and luciferase activity was measured. Results are the mean of triplicate experiments±s.d.