Fig. 8. Effect of cell permeable ceramides on FasL-induced cell death. (A) A20 or Raji cells (B) cells were incubated with 100 ng/ml FasL and C2-ceramide (100 µM) was added at time different time points after FasL. For comparison, cells were either not treated (NT or incubated with C2-ceramide (100 µM) or FasL (A20) or anti-Fas (Raji) (100 ng/ml) alone. For analysis, cells were harvested after 16 hours, stained with PI, and cell survival was determined by flow cytometric analysis (see Fig. 2). Means and standard deviations of data from three independent experiments are shown.