Fig. 2. GFP-Centrosomin localizes to centrosomes. GFP-Centrosomin fusion protein (GFP-Cnn) was expressed in the early embryo. This expression rescues the maternal effect lethal phenotype associated with cnn mutants and is therefore biologically active. These representative still images show GFP-Cnn localized to centrosomes (arrow in A), weakly to the spindle microtubules (arrowhead in A) and to punctate particles throughout the embryo. The stills show the first cycle in anaphase (A), late anaphase (B), telophase, when the centrosomes duplicate (C), prophase (D) and metaphase of the next cycle (E). The nuclei can be seen as the dark areas in D that are devoid of GFP-Cnn signal. See also Movie 1 at jcs.biologists.org/supplemental.