Fig. 6. Flares are dependent on microtubules. (A) GFP-Cnn embryos were injected with the microtubule-destabilizing drug colchicine to inhibit the formation of microtubules. Shown is a single still of the movie. Flare particles surround the centrosome but no longer move back and forth as they do in the untreated embryos. Incipient flares appear to emerge from the centrosome but do not bud off as flare particles. (B) Flares are associated with microtubules. Shown are images of embryos stained for Cnn (red), microtubules (green) and DNA (blue). Flare particles are often associated with microtubules (arrows in B), appearing at the end (solid arrow) of a microtubule (arrowhead) or projecting out like a tentacle along microtubules (arrow). The image in B was deconvoluted to resolve the Cnn and microtubule signals better. See also Movie 4 at jcs.biologists.org/supplemental.