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Fig. 3. Mitochondrio-nuclear AIF translocation in the interdigital cells of the mouse embryo. (A) Persistent interdigital web from an Apaf1-/- embryo (E15.5). The interdigital webs are marked by a dashed line. Holes are forming by Apaf1-independent death. The red insert is shown at higher magnification in B. (B) Double staining with Hoechst 33324 (blue) and an anti-AIF antibody (red). (C-E) Further magnification of the rectangle shown in B. Note that condensed nuclei (D) contain AIF (E) within the same cells (overlay in C). Arrows in C, D and E point to the same dying cells.