Fig. 3. Mutations in CKII consensus-motifs stabilize ß-catenin. (A) 293 cells stably transfected with either wt or Ser/Thr-mutant HA-ß-catenin were subjected to pulse-chase experiments as described in Materials and Methods. The triple-mutant used contained the three mutations indicated in panel A. Equal amounts of incorporated radioactivity were immunoprecipitated with anti-HA antibodies at the time points indicated and the autoradiographs were quantified by phospho-imager analysis. The half-life of Ser/Thr-mutant ß-catenin was approximately two- to threefold that of wt ß-catenin. (B) Quantitative measurements of band intensities from three independent experiments, using MacBas image analyzing software (circles, wild-type ß-catenin; squares, mutant ß-catenin).