Fig. 1. Localization of endogenous SARA on early endosomal membranes. Low magnification (A-C, Bar, 10 µm) and 4 selective views of high magnification (D-F, Bar, 2 µm) images obtained from a single confocal section (0.6 µm) of HEK cells double labeled for SARA (A,D) and EEA1 (B,E). The images reveal heterogeneous pools of early endosomes displaying various levels of SARA and EEA1. Some early endosomes have a higher level of SARA, while others have a higher level of EEA1. SARA and EEA1 did not always overlap on the membranes of individual early endosomes.