Fig. 3. Colocalization of acetylated histone H3 and acetylated histone H4 at lysines 8 and 12 in S. ocellaris embryonic germ nuclei. (A-D) DAPI staining, (A'-D') indirect immunolabeling with different antibody combinations that are specified in the top of the figure and (A''-D'') superimposed images where antibody staining is in red. In all cases the antibodies labeling is restricted to the same four chromosomes of the complement. Bar, 10 µm.