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Fig. 4. Distribution of acetylated histone H4 at lysine 8 in germ cells of S. ocellaris male larvae at one day (1d), five days (5d) and 10 days (10d) from hatching. (A-C) DAPI staining, (A'-C') indirect immunolabeling with H4Ac8 antibody and (A''-C'') superimposed images where antibody staining is in red. (A) Maternal chromosomes appear condensed (long arrows) whereas paternal chromosomes, less stained with DAPI, initiate decondensation (short arrows); (A',A'') H4Ac8-staining is restricted to the paternal chromosome set. (B) Maternal chromosomes remain condensed (long arrows) whereas paternal ones appear totally decondensed (short arrows); (B',B'') H4Ac8-labeling is restricted to the chromatin corresponding to the paternal chromosome set. (C) All chromosomes appear equally condensed and exhibit H4Ac8-staining (C',C''). Bar, 10 µm.