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Fig. 5. Distribution of acetylated histone H4 at lysine 8 in germ cells of S. coprophila male larvae at 3 days (3d) and 10 days (10d) from hatching. (A,B) DAPI staining, (A',B') indirect immunolabeling with H4Ac8 antibody and (A'',B'') superimposed images where antibody staining is in red. (A) Maternal chromosomes (long arrows) and L chromosomes (arrowheads) appear condensed and clustered whereas paternal chromosomes are fully decondensed (short arrows); (A',A'') Paternal chromatin exhibits H4Ac8-staining whereas maternal and L chromosomes are devoid of staining. (B-B'') All chromosomes are condensed and show H4Ac8-staining except the L chromosomes (arrowheads in B and B''). Bar, 10 µm.