Fig. 6. Distribution of acetylated histones in S. ocellaris (A-C) and S. coprophila (D) spermatocytes undergoing paternal chromosomes elimination at first meiotic division. (A-D) DAPI staining of anaphase-like stage figures where m refers to the maternal group of chromosomes, p refers to paternal chromosomes and ^* marks the position of the single spindle pole. (A'-D') Indirect immunolabeling with the antibody specified in the top of the figure and (A''-D''') superimposed images where antibody staining is in red. In all cases (A''-D''), the antibody labeling is restricted to the four maternal chromosomes that face the pole whereas the opposed paternal group of chromosomes is devoid of staining. Arrows in D indicate S. coprophila L chromosomes that migrate together with the maternal group of chromosomes and do not exhibit antibody labeling (D'-D''). Bar, 10 µm.