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Fig. 4. Phosphorylation of lasp-1 with PKA inhibits F-actin co-sedimentation. (A) Representative Coomassie blue-stained SDS-PAGE gel containing supernatants and pellets from an actin co-sedimentation assay. Arrow shows location of actin. Lanes 1,2,7,8: his-tagged lasp-1 alone; lanes 3,4,9,10: lasp-1 plus F-actin; lanes 5,6,11,12: phosphorylated lasp-1 + F-actin. (B) Scatchard analyses of lasp-1 and phosphorylated lasp-1 binding to F-actin. Values for each point represent averages from 4-5 independent experiments.