Fig. 7. In cultured gastric parietal cells, forskolin stimulation induces the recruitment of lasp-1 to the F-actin rich canalicular membrane region as well as to newly formed membrane extensions. Parietal cells in primary culture can be identified based on several different criteria including cell size and general morphology, autofluorescence when excited at lower wavelengths, lack of stress fibers or focal contacts, and the expansion of the canaliculi following secretagogue stimulation (Chew et al., 1989) (C.S.C., unpublished). (a,c,e,g) lasp-1; (b,d,f,h) F-actin. The cell in the top panel was treated with DMSO vehicle. Cells in the lower panels were stimulated with forskolin (10 µM, 30 minutes). Panels e-h contain images of the same cell acquired from the mid-section (e,f) and near the base (g,h). Arrows indicate position of expanded intracellular canaliculi. Arrowheads, newly formed cell extensions. Data representative of six independent experiments. Bars, 10 µM.