Fig. 9. In gastric mucosal fibroblasts, lasp-1 is recruited to regions of active membrane-cytoskeletal rearrangements following the activation of cAMP-independent signaling pathways. Gastric fibroblasts in mixed gastric mucosal primary cultures were growth-factor deprived for 24 hours, treated with 100 nM PMA (0 minutes, a-c; 2 minutes, d-f; 5 minutes, g-i; 10 minutes, j-l) and then fixed and stained for lasp-1 and F-actin as described in Materials and Methods and Fig. 5. Left panels, lasp-1; center panels, F-actin; right panels, merged images (lasp-1, red; F-actin, green). Arrows indicate locations of microspikes and nascent focal complexes. Data representative of three independent experiments. Bars, 5 µM.