Fig. 1. (A) Dot matrix analysis of dynein 2 HC. Rat dynein 2 HC sequence (database accession no. AB041881; abscissa) is compared with rat dynein 1 HC [ordinate for the top; accession no. L08505 (Mikami et al., 1993); accession no. D13896 (Zhang et al., 1993)] or C. elegans dynein 2 HC (ordinate for the bottom; F18C12.1, accession number Z75536). A schematic drawing of each gene product is given. The location of the six AAA (ATPases associated with a variety of cellular activities) modules (light brown boxes marked as A1 to A6) and P-loop motifs (in the 1st to 4th AAA modules only, shown as a red bar) is assigned on the basis of Neuwald et al. (Neuwald et al., 1999) using a sequence alignment. Coiled-coil prediction on the basis of a computer program (Lupas et al., 1991) is also shown as a blue histogram on top of each domain diagram. Locations of the three major stretches predicted to be coiled-coil are annotated as C1 to C3. In the box for the dynein 1 HC (top), the HC dimerization (HC), the IC-binding site (IC), and the LIC binding (LIC) sites (Tynan et al., 2000a) are given. Similar regions detected in dynein 2 and dynein 1 in the N-terminal third are shown in red in the top box (see Results). In the C. elegans dynein 2 HC, the hatched box shows the location of an exon on the genome sequence (reverse-complement sequence of nucleotides 8863-9000 in the Z75536 sequence), which has been ignored by the computer program for prediction and has been newly identified by the comparison with rat dynein 2 HC sequence. (B) Dot matrix analyses of LIC3 (ordinate; human CGI-60 sequence, accession no. AF151818) compared with rat LIC 2 [left box abscissa; accession no. U15138 (Hughes et al., 1995)] or C. elegans sequence F02D8.3 (C. elegans LIC3 in the right box abscissa; accession no. CAB01645). In mammalian LICs, the location of the P-loop motif is shown as a red bar.